Mouse Anti-Matrix Metalloproteinase 1 (MMP-1, Fibroblast/Interstital Collagenase-1)
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Applications
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: PLP fixed paraffin-embedded tissues required for best results. Western Blot: 1ug/ml Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa332-350 from human MMP-1.
Form
Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free).
Specificity
Recognizes the precursor and active forms of human MMP-1. Does not react with human MMP-2, 3, 8, 9, 13 and bovine MMP-1
