Mouse Anti-Matrix Metalloproteinase 9, 92kD (MMP-9, Collagenase 4, Gelatinase B)
MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and inhibition by specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs).The matrix metalloproteinases each have different substrate specificities within the ECM and are important in its degradation..
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Inhibition of MMP-9 activity. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1ug/ml for 2hrs at RT under non-reducing conditions. Immunohistochemistry (Acetone-fixed frozen): 1:100 for 30 minutes at RT. Immunoprecipitation: 2-5ug/mg protein lysate. Use Protein G. Inhibition: 2-4ug/ml Optimal dilutions to be determined by the researcher.
Positve Control
Conditioned serum-free medium from (dexamethasone-treated) human fibrosarcoma HT-10801 or endothelial HUVEC 4 cells.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human MMP-9. Cellular Localization: Peripheral cytoplasmic.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human MMP-9 at 92kD and 86kD (non-reducing conditions) which are identified as pro (latent) and active forms of matrix metalloproteinase-9 (MMP-9; also known as 92kD and collagenase IV or gelatinase B). Reacts with the complex (~200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1). Does not cross-react with pro and active forms of MMP-2.
