Technical Data

M2449-51A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Hu
Gene #
GNT2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MGAT2, CT (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannoside Acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, Beta-1,2-N-Acetylglucosaminyltransferase II, GlcNAc-T II, GNT-II)

MGAT2 (UDP-N-acetylglucosamine:alpha-6-D-mannoside-beta-1, 2-N-acetylglucosaminyltransferase II) is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in MGAT2 may lead to carbohydrate-deficient glycoprotein syndrome, type II.

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human MGAT2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MGAT2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Yen, C.L., et al., J. Biol. Chem. 278(20):18532-18537 (2003). 2.Chen, S.H., et al., Glycoconj. J. 15(3):301-308 (1998). 3.Tan, J., et al., Am. J. Hum. Genet. 59(4):810-817 (1996). 4.Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995). 5.D'Agostaro, G.A., et al., J. Biol. Chem. 270(25):15211-15221 (1995).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net