Goat Anti-MXD4 (Max Dimerization Protein 4, Max Dimerizer 4, Max-interacting Transcriptional Repressor MAD4, Max-associated Protein 4, Class C Basic Helix-loop-helix Protein 12, bHLHc12, MAD4)
MXD4 is a member of the MAD gene family . The MAD, basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 0.5-2ug/ml, observed in human kidney lysates on ~22kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to GPHCRRLGRPALS, from human MXD4, at C-terminal (NP_006445.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MXD4.
