Rabbit Anti-MCAM, ID (Cell Surface Glycoprotein MUC18, Melanoma-associated Antigen MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, S-endo 1 Endothelial-associated Antigen, Cell Surface Glycoprotein P1H12, CD146, MCAM, MUC18)
MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. It could be an adhesion molecule active in neural crest cells during embryonic development. It acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (FFPE): 1:50-1:100 Western Blot: 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa168-197 from the center region of human MCAM, conjugated to KLH. Cellular Localization: Membrane; SIngle-pass type 1 membrane protein
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human MCAM at ~71kD.
