Technical Data

M2775-15
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001003891.1; NP_056973.2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-MED 15 (Mediator complex subunit 15, Activator-recruited cofactor 105kD component, Arc105, ARC105, CAG7A, CTG7A, CTG repeat protein 7a, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, PCQAP, Positive cofactor 2 glutamine/Q-rich-associated protein, TIG1, TIG-1, TNRC7, TPA-inducible gene 1 protein)

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Applications
Sutable for use in ELISA and Western Blot. Other Applications not tested.
Recommended Dilutions
ELISA: 1:8000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher
Positive Control
Human Lung lysates
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See M2775-15-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
Synthetic peptide, C-PPQSQTQPLVSQAQ, from the internal region of human PCQAP.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human PCQAP at ~90kD. Species sequence homology: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Thakur JK, Arthanari H, Yang F, Pan SJ, Fan X, Breger J, Frueh DP, Gulshan K, Li DK, Mylonakis E, Struhl K, Moye-Rowley WS, Cormack BP, Wagner G, Näär AM. A nuclear receptor-like pathway regulating multidrug resistance in fungi. Nature 2008 Apr 452 (7187): 604-9.
USBio References
No references available
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