Technical Data

M2867-56L
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Accession #
BAA25025
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MAP3K7 (Mitogen-activated Protein Kinase Kinase Kinase 7, MEKK7, Transforming Growth Factor-beta-activated Kinase 1, TGF-beta-activated Kinase 1, TAK1, TGF1a)
EC=2.7.11.25

TAK1 was shown to participate in regulation of transcription by transforming growth factor-beta (TGF-beta). TAK1 is stimulated in response to TGF-beta and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF-beta superfamily members. TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF-b activated kinase 1) MAPKKK in TGF-b signal transduction. TAB1-induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1. TNF-a activated endogenous TAK1, and the kinase-negative TAK1 acted as a dominant negative inhibitor against TNF-a-induced NF-kB activation. TAK1 was suggested to act as a regulatory kinase of IKKs.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
NIH 3T3
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa19-35 and 564-579 of human TAK1 (accession number BAA25025).
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human TAK1/MAP3K7 (TGF-beta-activated kinase 1). Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Shibuya H, et al. Science 272(5265):1179-82 (1996). 2. Sakurai H, et al. Biochem Biophys Res Commun 243(2):545-9 (1998). 3. Sakurai H, et al. J Biol Chem 274(15):10641-8 (1999).
USBio References
No references available
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