Rabbit Anti-Melanocortin Receptor 2 (MC2-R, ACTH-R, a-MSH Receptor, MSH Receptor) (Not for Export EU)
Melanocortins are regulatory peptides formed by post-translational processing of pro-opiomelanocortin. Melanocortin peptides have been suggested to perform a variety of physiological roles ranging from control of behavior, memory, neurotrophic properties, antipyretic and modulation of immune system, etc. Their binding sites have been found distributed in tissues ranging from lachrymal and submandibular glands, pancreas, adipose tissue, bladder, duodenum, spleen, brain, gonadal tissues and malignant melanoma tumors. Five melanocortin receptors have been characterized to date. These include melanocyte-stimulating hormone receptor (MSH or MC1-R), corticoaderenal-specific ACTH receptor (MC2-R), melacortin-3 (MC3-R), melanocortin-4 (MC4-R) and melanocortin-5 receptor (MC5-R). MC3-R and MC4-R are distributed in brain whereas MC5-R has a broad distribution.
Mouse Melanocortin receptor (MC2-R or ACTH-R) is a 296 amino acid transmembrane protein belonging to the family of G-Protein coupled receptors. It is a receptor for ACTH. It is found in melanocyte and corticoadrenal tissues. Defects in MC2-R are the cause of familial glucocorticoid deficiency. An autosomal recessive disorder due to congenital insensitivity or resistance to ACTH and characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency.
Applications
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Anti-Mouse antibodies were raised against a 17 aa peptide from the 4th cytoplasmic domain of the murine MC2-R. The peptide was coupled with KLH (carrier protein)
Form
Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Specificity
The 17 AA mouse immunogenic peptide shows homology of with human (12/17 aa; 71%), golden hamster (13/17; 76%) and bovine (12/17; 70%). Actual crossreactivity of antibodies in all species is not established
