M3007-20
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
3i20(25H2)Grade
AscitesApplications
IP WBCrossreactivity
Hu Mk Mo RtGene ID
4233Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Met (Hepatocyte Growth Factor Receptor, HGF Receptor, Scatter Factor Receptor)
Met is a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor). Met is a disulfide-linked heterodimer made of 45kD alpha- and 145kD beta-subunits. The alpha-subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. This is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1. Altered Met levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Met is an attractive therapeutic target and cancer diagnostic.
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Tyr1234 of human Met.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of human Met at ~145kD and at 170kD for pro-Met. Species Crossreactivity: mouse, rat and monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Weidner, K.M., et al., J. Cell Biol. 121: 145-154 (1993). 2. Park, M., et al., Cell 45: 895-904 (1986). 3. Bardelli, A., et al., Oncogene 15: 3103-3111 (1997). 4. Taher, T.E., et al., J. Immunol. 169: 3793-3800 (2002). 5. Schaeper, U., et al., J. Cell Biol. 149: 1419-1432 (2000). 6. Traxler, P., et al., Med. Res. Rev. 21: 499-512 (2001).USBio References
No references available