Rat Anti-Meteorin
Meteorin is a 31kD, secreted protein that is expressed by undifferentiated neural and glial progenitors, and astrocytes. Its name derives from it ability to generate glial cells with long meteor-like tails. Mouse Meteorin is synthesized as a 291aa precursor that contains a 270aa mature region. It contains eight cysteines with no identifiable domains or N-linked glycosylation sites. There is one alternate splice form where the first 164aa of the precursor are deleted and aa165 is changed to a Met, possibly to serve in translation initiation. Mature mouse Meteorin is 82-77aa identical to human and bovine Meteorin, respectively.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 0.5-2ug/ml ELISA (capture): 2-8ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gly22-Asp291 from mouse Meteorin expressed in NSO cells.
Form
Supplied as a powder lyophilized in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse Meteorin. No cross-reactivity or interference with recombinant mouse CDNF, recombinant rat CNTF, and rrGDNF.
