Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemstry. Other applications not tested.

Immunohistochemistry (Paraformaldehyde (4%) fixed frozen): 1:10-1:20 Pretreat for 10 min with 20mM glycine in PBS, pH 7.5 and then for 10 min with 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5. Western Blot (ECL): 1:10-20 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

pET28a(+) expression vector containing aa408-611 of the p193 cDNA.

Supplied as a liquid, 1% BSA, 0.09% sodium azide.

Ascites

Recognizes an internal epitope (aa491-494, HPGE) of the human minor vault p193 protein. Overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.