Mouse Anti-Membrin (GS27, Golgi SNARE of 27K)
Membrin, also known as GS27 (Golgi SNARE of 27K), is a 27kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (1). GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1 (2,3). These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (2,3).
Applications
Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot (ECL): 2ug/mL Immunoprecipitation: 2-4ug/mL Immunocytochemistry: 1:500 (4) Optimal dilutions to be determined by the researcher.
Positive Control
HeLa Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat membrin fused to His6 tag
Form
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes rat Membrin. Species Crossreactivity: human, monkey, canine, chicken, hamster, porcine and rabbit
