Rabbit Anti-Monocarboxylate Transporter 5 (MCT5, MOT5)
Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
MCT5/SLC16A4 (mouse 468-aa, human 487-aa, chromosome 1p13.1) shares 25% sequence homology with MCT1, but it has much shorter C-terminus than other MCTs. MCT5 has an Alu insertion even in the 3'-UTR and a truncated C-terminus. High expression of MCT5 has been observed in placenta. According to new nomenclature, the old MCT4 has now been reclassified as MCT5.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:100,000 using 50–100ng control peptide per well. See Catalog #M4470-40B. Western Blot: 1-5ug/ml using ECL. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a 19aa sequence within the cytoplasmic domain #4, near the C-terminus of human MCT5 conjugated to KLH. Species Sequence Homology: mouse MCT5, 65%.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Monocarboxylate Transporter 5 (MCT5/MOT5). Reactivity with other members of the MCT family is unlikely because the immunogen shows no significant sequence homology with other MCTs.
