Technical Data

M4591-02A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)
Rabbit Anti-MOP3 (Members of PAS Superfamily, BMAL1, Brain And Muscle ARNT-like 1, JAP3, PAS3, CYCLE) (Not for Export EU)

Circadian rhythm is one of the most fascinating and complex biological phenomenon's. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules. Basic-helix-loop-helix-PAS orphan protein, MOP3 (Members Of PAS Superfamily; also known as BMAL1/JAP3/PAS3; 626 aa) is a general dimerization partner for several PAS superfamily of transcription regulators. MOP3 interacts with MOP4 (also known as NPAS2; 626 aa), Clock, HIF1a, and HIF2a. MOP4 is a brain specific homolog of Clock.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well, Cat #M4591-02B. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 15-aa peptide sequence within the C-terminus of human MOP.3 (Coupled to KLH).
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Purity
Serum
Specificity
Recognizes human MOP3. Species sequence homology from other proteins: rat- (TIC), human (bMAL1a, bMAL1b, PAS3, JAP3) and mouse (Arnt3).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hogenesch JB et al (1997) J Biol. Chem. 272, 8581-8593; Hogenesch JB et al (1998) PNAS 95, 5474 2. Wolting CD et al (1998) Mamm. Genome 9, 463.Takahata S et al (1998) BBRC 248, 789-794.
USBio References
No references available
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