Goat Anti-MTHFS (5-formyltetrahydrofolate Cyclo-Ligase, 5,10-Methenyl-Tetrahydrofolate Synthetase, Methenyl-THF Synthetase)
Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml, observed in human liver lysates on ~25kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to KRCLQHQEVKPYT, from human MTHFS, at the internal region of the protein (NP_006432.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MTHFS. Species sequence homology: Mouse.
