Goat Anti-MTR (Methionine Synthase, 5-methyltetrahydrofolate-Homocysteine Methyltransferase, Vitamin-B12 Dependent Methionine Synthase, MS)
Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 1-3ug/ml, HeLa and Jurkat cell lysates observed on ~150kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-VEKWLGPILGYDTD, from human MTR, at C-terminal (NP_000245.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MTR. Species sequence homology: Canine, mouse, porcine and rat.
