MUC4 has been found to be a heterodimeric glycoprotein complex that consists of ASGP-1 (ascites sialoglycoprotein-1), an O-glycosylated mucin subunit, and ASGP-2, an N-glycosylated transmembrane subunit. ASGP-1 and ASGP-2 is a multifunctional glycoprotein complex. ASGP-1, through its O-glycosylation of the tandem repeat domain, provides anti-recognition and anti-adhesive properties to tumor cells. ASGP-2 has two epidermal growth factor-like domains. ASGP-2 appears to be able to bind and modulate ErbB2 receptor phosphorylation, which allows rapid growth of tumor cells. Sialomucin complex (SMC, rat homologue of human MUC4) expression is found in normal secretory epithelial tissues in the adult rat, where the protein has multiple regulatory mechanisms. SMC is found in normal virgin mammary gland, elevated in the lactating gland, and further amplified in rat mammary adenocarcinoma. Transforming growth factor-beta (TGF-b) inhibits SMC expression. Abnormal expression of MUC4/SMC is found in several human epithelial tumors such as, breast, lung, cervix, and pancreas carcinomas. Unregulated MUC4 may promote tumor metastasis, which suggests that it has an important role in human tumor biology. For example, several studies indicate that the cytoplasmic tail of the transmembrane mucin is involved in cell-signalling events such as Sos-ras pathway, beta-catenin, and ErbB2 signalling pathway.

Sutiable for use in FLISA, Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Rat ASGP-2.

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography

Recognizes an epitope on the rat ASGP-2 subunit, which is one of the main subunits that comprise the MUC4 glycoprotein complex. Reactivity is confirmed with ASGP-2 transfected COS-7 cells and rat microvilli isolated from ascites cells of 13762 rat mammary adenocarcinoma. Species Crossreactivity: human and mouse.