Mouse Anti-MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin, TBM, Mucin 5AC, Gastric Mucin)
Mucins are high molecular weight glycoproteins that are heavily glycosylated with oligosaccharide side chains linked O-glycosidically to the protein backbone. At least nine mucin genes have been identified to date. The pattern of tissue and cell-specific expression of these mucin genes suggests a distinct role for each member of the diverse mucin family. In epithelial cancers, many of the phenotypic markers for pre-malignant and malignant cells have been found on the carbohydrate and peptide moieties of mucin glycoproteins. Deregulation of tissue and cell-specific expression of mucin genes has also been described in epithelial cancers. At present, the role of mucin glyoproteins in various states of epithelial cell carcinogenesis, including the pre-neoplastic state and metastasis, is under investigation. The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen/paraffin): 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Recommended Positive Controls
Normal human epithelial tissues: bronchus, endocervix, stomach and gallbladder
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the MUC5AC tandem repeat.
Form
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Specificity
Recognizes the MUC5AC tandem repeat of human mucin. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa.
