Mouse Anti-Mucin 5AC (MUC5AC, Apomucin, Gastric Mucin, Major Airway Glycoprotein, MUC5, Mucin 5 Subtype AC Tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5 Subtypes A and C Tracheobronchial/gastric, Mucin 5AC Oligomeric Mucus/gel Forming, Tracheobronchial Mucin, TBM, Lewis B Blood Group Antigen, LeB)
Mucins are high molecular weight glycoproteins that are found especially in the secretions of mucus membranes. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 2-4µg/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mucin from human ovarian cyst fluid.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Mucin Gastric.
