Mouse Anti-Muscleblind-like 1 (MBNL1, EXP35, EXP40, EXP42, Muscleblind-like, MBNL, MBNL Protein, Muscleblind 41kD Isoform, Triplet Expansion RNA Binding Protein, DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428)
Several important human diseases are associated with expansion of polynucleotide sequences, in most cases trinucleotide repeats. Myotonic dystrophy (DM1) is one of these diseases, and is associated with increases in the number of CTG repeats in the 3' UTR of the gene encoding myotonin (a.k.a. DM Kinase, DMK or myotonin-Protein Kinase), a ser/thr kinase expressed specifically in muscle. Mammalian genomes contain three muscleblind-like proteins, generally referred to as MBNL1, MBNL2 and MBNL3, and all three were found to associate with long double stranded CUG RNA repeats, thus, being triplet repeat expansion dsRNA-binding proteins.
Applications
Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.2-0.5ug/ml using HeLa nuclear cell extract Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant fusion protein corresponding to human muscleblind-like1 (MBNL1).
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity
Recognizes human Muscleblind-like 1. Species Crossreactivity: mouse and monkey
