Rabbit Anti-mutY
Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity. The post-replicative adenine:8-oxo-7,8- dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion. This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO. A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1).
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications have not been tested.
Recommended Dilution
ELISA: 1:10,000-50,000. Using 50-100ng control peptide/well. Western Blot: 1:1000-1:5000. Optimal dilutions to be determined by the researcher.
Control Peptide: M9555-01
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 19-aa peptide near the C-terminus of human mutY (KLH).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human mutY at ~55kD. The human peptide sequence has no significant homology with E. coli mutY.
