Goat Anti-MxI1 (MAX Interactor 1, MAX-interacting Protein 1)
Mxi-1 (Max interactor 1) is a 26kD member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif (aa 69-80) and a bHLH domain (aa 81-120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81-228, human Mxi-1 is 95% aa identical to mouse Mxi-1.
Applications
Suitable for use in Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 10ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived, recombinant human Mxi1 (aa81-228) UniProt # P50539
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MXI1.
