Mouse Anti-Myc-Tag (c-myc, myc-1 epitope tag) (Biotin)
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-1ug/ml detects Myc-tagged recombinant protein. COS transfected cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-myc tag, biotin conjugate (1ug/ml). Proteins were visualized using a streptavidin-HRP conjugate and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to aa410-420 (MEQKLISEEDL) of human Myc.
Form
Supplied as a liquid in PBS, 0.05% sodium azide before the addition of glycerol to 30%. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. Species Crossreactivity: Human. Other species crossreactivity not tested.
