Rabbit Anti-Myc-Tag (c-myc, myc-1 epitope tag) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunoblot: 0.5-2ug/ml of this lot detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate. 1ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract. A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Myc epitope tagged protein (~66kD) Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN)
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
