Technical Data

M9758-01P
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Myelin Basic Protein (MBP, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675)

Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of ~80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (~75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunohistochemistry (Formalin fixed, paraffin): 1ug/ml Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa289-304, KLGGRDSRSGSPMARR, from the C-terminus of human Myelin Basic Protein (MBP). This sequence also corresponds to a region on rat MBP (aa180-195). Species Sequence Homology: mouse.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Myelin Basic Protein at ~33kD. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Schwartz, et al., Prog Brain Res 137, 401-6 (2002)|Hauben, et al., J Clin Invest 108, 591-9 (Aug, 2001)|Yoles, et al., J Neurosci 21, 3740-8 (Jun 1, 2001)|Hauben, et al., J Neurosci 20, 6421-30 (Sep 1, 2000)|Harauz, et al., Nature 389, 783-4 (1997). Micron 35, 503-42 (2004)|Givogri, et al., J Neurosci Res 59, 153-9 (Jan 15, 2000)|Kim, et al., Int J Biochem Cell Biol 29, 743-51 (May, 1997)|Kalwy, et al., Mol Membr Biol 11, 67-78 (Apr-Jun, 1994)|Wajgt, et al., Acta Neurol Scand 68, 337-43 (Nov, 1983)|Day, et al., J Neuroimmunol 10, 289-312 (Feb, 1986)|1Mikoshiba, et al., Comp Biochem Physiol C 98, 51-61 (1991)|1Brophy, et al., Trends Neurosci 16, 515-21 (Dec, 1993)|1Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
USBio References
No references available
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