Rat Anti-Myelin Basic Protein, aa82-87 (MBP)
Applications
Suitable for use in Immunohistochemistry, ELISA, RIA and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
Goat Anti Rat IgG: HRP (mouse absorbed)
Fusion Partners
Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid, 0.1M TRIS, 0.09% sodium azide.
Purity
Tissue culture supernatant.
Specificity
Recognizes bovine myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 (12) has been reported as being suitable for use in Western blotting. Species Crossreactivity: Reacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3).
