Technical Data

M9759-05A
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
ER-MP58
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Myeloid (Precursor) Cells

The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting,

Applications
Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (frozen): 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Myeloid (Precursor) Cells.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Cook, A et al; The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation. Thromb Haemost 2006, 95: 659 |2. Sunderkötter, C et al; Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J Immunol 2004, 172: 4410 3. Nikolic, T et al; Developmental stages of myeloid dendritic cells in mouse bone marrow. Int Immunol 2004, 15: 515 4. Chan, J et al; Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression. Blood 1998, 92: 1423 5. De Bruijn, M et al; High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage. Eur J Immunol 1996, 26: 2850 6. Leenen, P et al; Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol 1990, 20: 27
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