Mouse Anti-Myeloperoxidase (MPO, pANCA) (PE)
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry (Neat): Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Detects myeloid cells following membrane permeabilization. Optimal working dilutions to be determined by researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative Controls
I1904-79D IgG1 Murine Negative Control (PE)
Storage and Stability
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Immunogen
Human myeloperoxiase.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils.
