Mouse Anti-MyoD1 (Myogenic Differentiation 1, Myoblast Determination Protein 1, MYOD, Myogenic Factor 3, MYF3, MYF-3, PUM)
MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (FFPE): 1:20-1:50. May be used in an ABC method. Requires heat mediated antigen retrieval. Incubate 30-60 minutes at RT. Immunohistochemistry: 1:10-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to full length mouse MyoD1 protein.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, Tween-20, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes an epitope of aa 3-56 in the N-terminus of the 45kD mouse MyoD1 protein. Does not react with myogenin, Myf5 or Myf6. Stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas. |Species Crossreactivity: human, rat and chicken
