Technical Data

M9875-02N
Clone Type
Polyclonal
Host
Goat
Source
Human
Conjugate
Biotin
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-Myostatin (MSTN, Growth Differentiation Factor 8, Growth/Differentiation Factor 8, GDF8, GDF-8) (Biotin)

GDF­8, also known as Myostatin, belongs to the TGF-beta superfamily. It is synthesized as an inactive proprotein with the N­terminal pro­region and the C­terminal mature bioactive region. The GDF­8 proregion is capable of associating with active GDF­8 with high affinity and is a potent GDF­8 antagonist.

Applications
Suiitable or use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml Immunohistochemistry (Frozen): 5-15ug/ml using immersion fixed frozen sections of mouse embryo and mouse embryonic lung. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa268-376 from mouse GFD-8/Myostatin (O08689), expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 50ug BSA/1ug. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Myostatin. Species Crossreactivity: mouse, rat. In Western Blot ~10% crossreactivity is observed with recombinant mouse GDF-3 and <2% with recombinant mouse GDF-1,-5 -6.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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