Rabbit Anti-Myotubularin Related Protein 4, CT (MTMR4, FYVE Domain-containing Dual Specificity Protein Phosphatase 2, FYVE-DSP2, KIAA0647, Zinc Finger FYVE Domain-containing Protein 11, ZFYVE11)
Myotubularin-related Protein 4, EC=3.1.3.48
MTMR, a member of the myotubularin family, encodes an intracellular protein of 1,195 amino acids, and shares approximately 47% sequence identity with MTMR3, spiking to over 70% identity in the catalytic domain and the double zinc finger (FYVE) motif. PCR analysis detects expression of MTMR4 in all human tissues examined except lung, small intestine, stomach, salivary gland, adrenal gland, and uterus. Subcellular localization of MTMR4 in transfected HeLa cells showed a perinuclear distribution, similar to that seen with MTMR3. MTMR4 dephosphorylates para-nitrophenylphosphate and phosphatidylinositol 3-phosphate.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1155-1187 in the C-terminal region of human Myotubularin Related Protein 4.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human Myotubularin Related Protein 4. Species Crossreactivity: mouse
