Technical Data

N0002-85
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_005958
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NAB2 (NGFI-A Binding Protein 2, EGR-1-binding Protein 2, Melanoma-associated Delayed Early Response Protein, MADER, Protein MADER, MGC75085)
NGFI-A-binding Protein 2 (EGR1 Binding Protein 2)

NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by this protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a portion of amino acids within aa116-146 from the Center region of human NAB2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human NAB2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Srinivasan,R., J. Biol. Chem. 281(22), 15129-15137 (2006). 2.Kumbrink,J., J. Biol. Chem. 280(52), 42785-42793 (2005). 3.Lucerna,M., J. Biol. Chem. 278(13), 11433-11440 (2003).
USBio References
No references available
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