N0009-40U
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo Rb RtAccession #
NP_004997.4Gene ID
4719, 227197, 301458Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-NDUFS1 (NADH-ubiquinone Oxidoreductase 75kD Subunit, Mitochondrial, Complex I-75kD, CI-75kD)
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 0.3-1ug/ml, heart and mouse heart lysates observed on ~80kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TEKSATYVNTEGR, from NDUFS1, at the internal region of the protein (NP_004997.4).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NDUFS1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, rabbit and rat.
