N0018-01K
Clone Type
MonoclonalHost
RabbitSource
HumanIsotype
IgGClone Number
11C1052(D22G5)Grade
PurifiedApplications
WBCrossreactivity
HuGene ID
3164Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-NAK1, phosphorylated (Ser351) (NAK-1, Early Response Protein NAK1, GFRP1, HMR, MGC9485, NGFIB, N10, NP10, Nuclear Hormone Receptor NUR/77, Nur77, Nuclear Receptor Subfamily 4 Group A Member 1, NR4A1, Orphan Nuclear Receptor HMR, Orphan Nuclear Receptor TR3, ST-59, Testicular Receptor 3, TR3)
Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily. Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxyterminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals. It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis. Nur77 binds to specific DNA elements leading to the regulation of target genes. As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL. Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization. Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis. Phosphorylation of Nur77 by Akt or RSK occurs at Ser351 (corresponding to rat Nur77 Ser350 and Ser354 of mouse Nur77), a site within the Nur77 DNA binding domain. Serine phosphorylation at this site can down regulate transcriptional activity of Nur77.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser351 of human NAK1. Species Sequence Homology: mouse and rat.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of human NAK1, phosphorylated at Ser351.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85,8444-8448. 2. Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977. 3. Milbrandt, J. (1988) Neuron 1, 183-188.. 4. Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459. 5. Liu, Z.G. et al. (1994) Nature 367, 281-284. 6. Woronicz, J.D. et al. (1994) Nature 367, 277-281. 7. Wilson, T.E. et al. (1991) Science 252, 1296-1300. 8. Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538. 9. Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536. 10. Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812. 11. Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246. 12. Li, H. et al. (2000) Science 289,1159-1164. 13. Lin, B. et al. (2004) Cell 116, 527-540. 14. Pekarsky, Y. et al. (2001) Proc. Natl. Acad. Sci. U S A 98, 3690-3694. 15. Han, Y.H. et al. (2006) Oncogene 25, 2974-2986. 16. Wingate, A.D. et al. (2006) Biochem. J. 393, 715-724. 17. Katagiri, Y. et al. (1997) J. Biol. Chem. 272, 31278-31284.USBio References
No references available