Rat Anti-Natriuretic Peptide, C-Type (CNP)
C-Type Natriuretic Peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49aa propeptide and a 53aa mature peptide. Additional cleavage of the mature peptide generates 29aa and 22aa peptides which have comparable activity in some assays. Within the 53aa mature CNP, human shares 96% aa sequence identity with mouse and rat.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1ug/ml using recombinant human C-type Natriuretic Peptide Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Lys24-Cys126 of human Natriuretic Peptide, C-Type expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human pro-CNP and mature CNP53 and CNP29. Does not crossreact with recombinant human ANP or BNP.
