Rabbit Anti-Nav1.7 (PN1, Type IX Voltage-gated Sodium Channel, NENA, NE, Scn9a)
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot (ECL): 1:200. Rat brain membranes Immunohistochemistry: Frozen rat dorsal root ganglion. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal dilutions to be determined by the researcher.
Control Antigen Included
N0530-25A: 40ug of lyophilized peptide MW: 1885D
Preadsorption Control
1ug peptide per 1ug antibody.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa446-460, C-EFTSIGRSRIMGLSE, of rat Nav1.7. Epitope location: Intracellular loop between I and II domains. Species sequence homology: rabbit,14/15 and human -13/15.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile ddH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes rat Nav1.7. The epitope is specific for Nav1.7, Pain, and is not present in any other known protein.
