Technical Data

N0531-ML650
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
MaxLight™650
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene ID
342538
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rabbit Anti-NAC, alpha (Nascent polypeptide-associated complex) (MaxLight 650)

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Nascent polypeptide-associated complex (NAC) is an abundant cytosolic protein involved in cotranslational targeting of polypeptides to the endoplasmic reticulum (ER) membrane. NAC preparations consist of two copurifying polypeptides termed alpha- and beta-NAC that form a very stable complex in vivo and in vitro, and a functional complex can be reconstituted from the individual subunits. A dissection of the contributions of the individual subunits to NACs function revealed that both subunits are in direct contact with nascent polypeptide chains on the ribosome and that both contribute to the prevention of inappropriate interactions. Alpha-NAC is a coactivator of AP-1-mediated transcription. The alpha-NAC protein functions as a transcriptional coactivator by potentiating the activity of the chimeric Gal4-VP16 activator and of c-Jun homodimers. alpha-NAC binds to and regulates FADD function, which is a critical mediator of signal transduction pathways activated by several members of the TNF receptor gene superfamily. Human alpha-NAC is decreased in patients with Alzheimer’s disease and Down syndrome.
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Controls
Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
C-terminal region of human alpha-NAC
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the human alpha-NAC protein at ~23kD. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Beatrix, B., et al., J. Biol. Chem. 275: 37,838-37,845 (2000). 2. Moreau, A., et al., Mol. Cell Biol. 18: 1312-1321 (1998). 3. Quelo, I., et al., Biochemistry 43: 2906-2914 (2004). 4. Quelo, I., et al., Gene Expr. 10: 255-262 (2002). 5. Yotov, W.V., et al., Mol. Cell Biol. 18: 1303-1311 (1998). 6. Stilo, R., et al., Biochem. Biophys. Res. Commun. 303: 1034-1041 (2003). 7. Kim, S.H., et al., J. Investig. Med. 50: 293-301 (2002).
USBio References
No references available
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