Technical Data

N0574-80B
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_690869.1
Gene ID
121441
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-NEDD1 (Protein NEDD1, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 1, NEDD-1)

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 1-3ug/ml, observed in lysates of cell line HeLa on ~75kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NQTRNSEKFEKPEN, from human NEDD1, at the internal region of the protein (NP_690869.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NEDD1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15. Epub 2006 Feb 6.
USBio References
No references available
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