N0800-07Q
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo RtAccession #
NP_598001.1Gene ID
140609, 59125, 360850Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-NEK7 (NIMA, (Never In Mitosis Gene A)-Related Kinase 7, NIMA-Related Kinase 7)
NEK7 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. NEK7 expression has been documented in normal human blood, brain, heart, kidney, liver, lung, ovary, prostate, skeletal muscle and testis. ESTs have been isolated from several tissue libraries, including normal adrenal, bone, pancreas and skeletal muscle and cancerous blood, head/neck, ovary and lung.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 0.05-0.2ug/ml, lysates of cell line HeLa observed on ~35kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NTLANFRIEKK, from human NEK7, at the internal region of the protein (NP_598001.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NEK7. Species sequence homology: Bovine, canine, mouse and rat.
