Rat Anti-Netrin G2a
Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59aa and 34aa between EGF-like motifs 1 and 2 generate Netrin G2b and Netrin G2c, respectively. Mature mouse Netrin G2a shares 96% and 99% amino acid identity with human Netrin G2 and the equivalent region in rat Netrin G2b, respectively.
Applications
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunohistochemistry: 25ug/ml using perfusion fixed frozen sections of mouse brain (hippocampus) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Asp18-Arg504 from mouse Netrin G2a expressed in NSO cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4.
