Rabbit Anti-Neurotrypsin (Leydin, Motopsin)
Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation (1-3). It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala (1). Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator (3). At least two isoforms of neurotrypsin are known to exist.
Applications
Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1 ug/ml. Optimal dilutions to be determined by the researcher.
Positive Control
SK-N-SH cell lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
23 amino acid peptide from near the amino terminus of human neurotrypsin.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human neurotrypsin. Species Crossreactivity: Mouse and rat.
