Rabbit Anti-Neurosecretory protein VGF (Neuroendocrine Regulatory Peptide-1, NERP-1, Neuroendocrine Regulatory Peptide-2, NERP-2, VGF)
VGF encodes a 70kD polypeptide that shares similarities with the secretogranin/ chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. Expression of VGF is both developmentally regulated and in regulated in different brain regions in response to different stimuli. VGF has been shown to play a role in energy homeostasis. Expression of VGF in the hypothalamus of rats and mice is induced by exercise and in response to fasting.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17aa near the C-terminal of the human VGF.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human VGF. Species sequence homology: mouse and rat
