Technical Data

N1256-15B
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Neurokinin B (Neurokinin-B, Neurokinin beta, NKB, NKNB, Gamma Tachykinin 3, Neurokinin B-like Protein, Neuromedin K, Neuromedin-K, Preprotachykinin B, Preprotachykinin-B, PRO1155, Tachykinin 3, Tachykinin-3, TAC3, ZNEUROK1)

proNeurokinin B (proNKB) is a neuropeptide belonging to the Tachykinin family, widely expressed in the Central Nervous System (CNS). It plays a neuromodulatory role in various brain functions.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:500-1:1000 for human hypothalamus and rat brain sections where positive staining of the arcuate nucleus is observed. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to an internal sequence of amino acids of the mouse proNKB protein (~93% homology to the rat sequence).
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Neurokinin B. Species Crossreactivity: human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Marksteiner, J., et al. Disribution of Neurons Expressing Neurokinin B in Rat Brain: Immunohistochemistry and In situ Hybridization. J. of Compar. Neur. 317: 341-356 (1992). 2. Mileusnic, D., et al. Neurokinin-3 Receptor Distribution in Rat and Human Brain: An Immunohistochemical Study. Neurosci. 89:1269-1290 (1999). 3. Goubillon, ML., et al. Identification of Neurokinin B-Expressing Neurons as an Highly Estrogen-Receptive, Sexually Dimorphic Cell Group in the Ovine Arcuate Nucleus. Endocr. 141: 4218-4225 (2000). 4. Severini, C., et al. A Tachykinin-like Factor Increase Glutamate Toxicity in Rat Cerebellar Granule Cells. Neuropharm. 44:117-124 (2003).
USBio References
No references available
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