Rabbit Anti-NHE5 (Na, H Exchanger 5) (Not for Export EU)
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, and regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
NHE5 (chromosome 16q22.1) is expressed in human brain, testis, spleen and skeletal muscle. It is involved in pH regulation to eliminate acids generated by active metabolism. It is a major proton extruding system driven by the inward sodium ion chemical gradient (1).
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 1:100,000 using 50-100ng control peptide/well. Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Control Peptide
Catalog #N2019-07: NHE5, Control Peptide, Human (Na, H Exchanger 5)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A 13aa peptide within the N-terminal cytoplasmic domain of rat NHE5 (KLH). |Species Sequence Homology: mouse, rat and porcine: 100%.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity
Recognizes human NHE5 at ~100kD. No significant homology exists with other NHE isoforms (NHE1-4).
