Rabbit Anti-Nerve Growth Factor, beta (NGF, Beta Nerve Growth Factor, Beta Nerve Growth Factor Precursor, Beta NGF, HSAN5, Nerve Growth Factor beta, Nerve Growth Factor beta Polypeptide, Nerve Growth Factor beta Subunit, NGFB)
Nerve Growth Factor is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested.
Recommended Dilutions
ELISA: 1-3ug/ml Western Blot: 1-3ug/ml Immunohistochemistry (frozen): 1-3ug/ml Inhibition of Biological Activity In vitro: 2ug/ml will neutralize 100ng/mL of mouse NGF. The higher 10ug/mL is only recommended if the concentration of NGF being used is higher than 100ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems Optimal dilutions to be determined by the researcher.
Biological Activity
N2050-06F completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro. Use neat for in vivo studies at 2-10ug/ml (ED50).
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE).
Form
Supplied as a lyophilized powder. Reconstitute in 500ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse nerve growth factor-beta (NGF). Crossreactivity: <1% against recombinant human BDNF, NT3 and NT4 by ELISA. Species Crossreactivity: rat, avian and human. Does not react with bovine NGF.
