Rabbit Anti-Neuron derived neurotrophic factor (NENF, neudesin, CIR2, SPUF, SCIRP10)
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control: Human Small Intestine Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to15aa from near the carboxy terminus of human NENF.
Form
Supplied as a liquid in PBS containing 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NENF. Species Crossreactivity: Mouse and rat.
