Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine- Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP- A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP- C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon- 1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non- SCLC (cell cultures). Suitable for use in Immunohistochemistry, Immunocytochemistry, Flow Cytometry, and Western Blot. Other applications not tested.

Immunohistochemistry (Frozen/paraffin): 1:100-1:200, using avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Flow Cytometry: 1:100-1:200 Western Blot: 1:100-1:500 Optimum dilutions to be determined by researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Neuroendocrine Specific Protein AB

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

Purified by Protein G affinity chromatography.

Recognizes human Neuro-endocrine specific proteins (NSP) A and B. Recognizes an epitope located within the region of aa421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining. Species crossreactivity: rabbit, monkey