Mouse Anti-Neurofilament Light (NF-L, NF68, NEFL, Neurofilament light polypeptide, NFL)
Neurofilaments are composed of three intermediate filament proteins: light (68kD), medium (160kD) and heavy (200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (formalin, paraffin, acetone): 2.0-4.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Porcine spinal cord.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity
Purified by affinity chromatography.
Specificity
Recognizes dephosphorylated porcine NF68. Species Crossreactivity: human.
