Chicken Anti-Neurofilament, Heavy Chain (NF-H)
Human Neurofilament Heavy Chain (NF-H) is a 115.378kD protein (1072aa) that forms part of the neuronal cytoskeleton. NF-H is physically associated with 10nm intermediate filaments, and is said to be one of the “neurofilament triplet” proteins, although it probably serves more to stabilize the cytoskeleton, rather than to be an integral structural component. NF-H immunoreactivity is found in neuronal somata, dendrites and axons.
Applications
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000:1:5000 using 2% paraformaldehyde tissues Immunocytochemistry: 1:2000:1:5000 using 2% paraformaldehyde cells Western Blot: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified neurofilament protein (200kD) isolated from bovine brains.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity
Recognizes Neurofilament, Heavy Chain. Species Crossreactivity: human, mouse and rat.
