Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques, such as Golgi staining, and modern molecular approaches, such as GFP constructs, yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, an Alexa 488® conjugated version of the pan-neuronal antibody blend was created to facilitate immunofluorescence studies. Each antibody within the blend is conjugated to Alexa 488® therefore enabling detection of an entire neuron with only one microscopic emission channel. This antibody mixture has been validated in diverse fixations, cell culture, and immunohistochemistry protocols, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology. High morphological resolution has been achieved, including distral dentrites and spines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.